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The Kjeldahl method or Kjeldahl digestion (Danish pronunciation: [ˈkʰelˌtɛˀl]) in analytical chemistry is a method for the quantitative determination of a sample's organic nitrogen plus ammonia/ammonium (NH 3 /NH 4 +). Without modification, other forms of inorganic nitrogen, for instance nitrate, are not included in this measurement.
Johan Gustav Christoffer Thorsager Kjeldahl (Danish pronunciation: [joˈhæn ˈkʰelˌtɛˀl] 16 August 1849 – 18 July 1900), was a Danish chemist who developed a method for determining the amount of nitrogen in certain organic compounds using a laboratory technique which was named the Kjeldahl method after him.
The NSI is the amount of Nitrogen in this filtered solution divided by the nitrogen in the initial sample, as measured by the Kjeldahl method. [ 3 ] The relevance of the NSI is based on the fact that proteins are the major biological source of Nitrogen: for various types of protein, there are empirical formulas which correlate the nitrogen ...
Protein concentrations of the defatted powdered immunogens are set through the Kjeldahl method with 46.4% raw defatted walnut and 34.9% roasted defatted walnut. [ 37 ] Polyclonal antibody production and titer determination
Protein methods are the techniques used to study proteins. There are experimental methods for studying proteins (e.g., for detecting proteins, for isolating and purifying proteins, and for characterizing the structure and function of proteins, [ 1 ] often requiring that the protein first be purified).
This score means, after digestion of the protein, it provides per unit of protein 100% or more of the indispensable amino acids required. The formula for calculating the PDCAAS percentage is: (mg of limiting amino acid in 1 g of test protein / mg of same amino acid in 1 g of reference protein) x fecal true digestibility percentage. [2]
Application of the Method of Continuous Variation, otherwise known as the Job plot, with the solution hydrodynamic radius as the observable, enables in vitro determination of K d, complex stoichiometry, complex hydrodynamic radius, and the ΔH° and ΔS° of protein–protein interactions. [16] This technique does not entail immobilization or ...
Kjeldahl may refer to: Johan Kjeldahl (1849–1900), Danish chemist; Kjeldahl method, analytical chemistry method for determining total nitrogen