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The binding of low molecular ... Thermostable proteins are often more useful than their non-thermostable counterparts, e.g., DNA polymerase ... Test ligands are ...
The synthesis rate of Taq polymerase is around 60 base pairs per second. Among the unmodified thermostable DNA polymerases, only the synthesis rate of KOD polymerase is above 100 base pairs per second (approx. 120 bp/s). [11] Among the modified thermostable DNA polymerases, various mutations have been described that increase the synthesis rate.
A previous study looked at genetic variants in the genomes of dogs near the abandoned plant, identifying 391 outlier DNA segments that differed between two populations.
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by master's student Alice Chien et al. in 1976. [1]
The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the DNA-binding domain (DBD or often also abbreviated as BD) and activating domain (AD).
Target DNA fragments (or cDNA) are first inserted into a cloning vector, and a single set of primers are designed for the areas of the vector flanking the insertion site. Amplification occurs for whatever DNA has been inserted. [4] PCR can easily be modified to produce a labeled product for subsequent use as a hybridization probe. One or both ...
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