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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
The decay scheme of a radioactive substance is a graphical presentation of all the transitions occurring in a decay, and of their relationships. Examples are shown below. It is useful to think of the decay scheme as placed in a coordinate system, where the vertical axis is energy, increasing from bottom to top, and the horizontal axis is the proton number, increasing from left to right.
The graph shown in figure 1 is a very simple version of what a graph could look like. [2] This graph is formed by taking the last 6 elements of the 7-mer and linking it to the node whose first 6 elements are the same. Figure 1 is the most simplistic a de Bruijn graph can be, since each node has exactly one path into it and one path out. Most of ...
Pyrosequencing, a new method of DNA sequencing which involves the addition of phosphorylated dNTP's to the DNA polymerase reaction which emmit light upon binding to the DNA template. It makes DNA sequencing significantly faster than with the current chain termination method.
Longer read lengths: ZS Genetics has estimated potential read lengths of transmission electron microscopy DNA sequencing to be 10,000 to 20,000 base pairs with a rate of 1.7 billion base pairs per day. [7] Such long read lengths would allow easier de novo genome assembly and direct detection of haplotypes, among other applications. [11]
It uses 2 base encoding to decode the raw data generated by the sequencing platform into sequence data. This method should not be confused with "sequencing by synthesis," a principle used by Roche-454 pyrosequencing (introduced in 2005, generating millions of 200-400bp reads in 2009), and the Solexa system (now owned by Illumina) (introduced in ...
A ternary plot, ternary graph, triangle plot, simplex plot, or Gibbs triangle is a barycentric plot on three variables which sum to a constant. [1] It graphically depicts the ratios of the three variables as positions in an equilateral triangle .
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