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The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. [2] [3] Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks.
The notion of doubling time dates to interest on loans in Babylonian mathematics. Clay tablets from circa 2000 BCE include the exercise "Given an interest rate of 1/60 per month (no compounding), come the doubling time." This yields an annual interest rate of 12/60 = 20%, and hence a doubling time of 100% growth/20% growth per year = 5 years.
Immunological synapse between Jurkat T cell expressing GFP-actin (green) and Raji B cell stained with CMAC (blue). Synapse formation was induced by Staphylococcal enterotoxin E superantigen . In immunology , an immunological synapse (or immune synapse ) is the interface between an antigen-presenting cell or target cell and a lymphocyte such as ...
The average doubling time is 19 to 50 hours. 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) are also commonly added to inhibit bacterial contamination. Cultures should be maintained at cell densities in the range 2-9x10 5 cells/ml at 37 °C, 5% CO 2. Cells are non-adherent. [5]
It is expressed on all leukocytes but highest on thymocytes [7] [8] [9] and is believed to augment T-cell adhesion [10] [11] and apoptosis of double positive T cells. [12] It has been found in endothelial cells and in the periodontium, including gingival fibroblasts and gingival epithelial cells. [5] It also participates in migration and ...
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Cell growth refers to an increase in the total mass of a cell, including both cytoplasmic, nuclear and organelle volume. [1] Cell growth occurs when the overall rate of cellular biosynthesis (production of biomolecules or anabolism) is greater than the overall rate of cellular degradation (the destruction of biomolecules via the proteasome, lysosome or autophagy, or catabolism).
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