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  2. Suspension culture - Wikipedia

    en.wikipedia.org/wiki/Suspension_culture

    CHO cells in suspension. A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The ...

  3. Dilution cloning - Wikipedia

    en.wikipedia.org/wiki/Dilution_cloning

    Dilution cloning or cloning by limiting dilution [1] [2] describes a procedure to obtain a monoclonal cell population starting from a polyclonal mass of cells. This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made. Appropriate dilutions are then made ...

  4. Trypsinization - Wikipedia

    en.wikipedia.org/wiki/Trypsinization

    When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel. Trypsinization is often used to pass cells to a new vessel. When the trypsinization process is complete the cells will be in suspension and appear rounded.

  5. Adherent culture - Wikipedia

    en.wikipedia.org/wiki/Adherent_Culture

    The culture can be visualized using an inverted microscope, however the growth of adherent cultures is dependent on the available surface area. For this reason, adherent cell cultures are not commonly used to obtain a high yield of cells, instead the use of suspension cultures is preferred. [4]

  6. Microcarrier - Wikipedia

    en.wikipedia.org/wiki/Microcarrier

    Microcarrier cell culture, however, was the breakthrough required for cell culture to reach industrial and clinical significance. [2] Studies have shown that microcarrier suspensions, compared to multi-layer vessel culture, improve cell yield by 80-fold at only ten percent of Good Manufacturing Practice space, and only sixty percent of the ...

  7. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...

  8. Virtual colony count - Wikipedia

    en.wikipedia.org/wiki/Virtual_colony_count

    Adhesion, causing cells to stick to the microplate and possibly form biofilms. Unless these cells happen to be directly in the light path, their growth will not affect optical density readings. Cohesion, causing cells to aggregate into clumps of various sizes instead of a homogeneous suspension of individual cells undergoing binary fission.

  9. Subculture (biology) - Wikipedia

    en.wikipedia.org/wiki/Subculture_(biology)

    In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. [1]