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Both qualities are inversely correlated with resolution: the higher the resolution (i.e. the greater the distance at which two adjacent points can be distinguished as separate), the smaller the depth of field and working distance. Some stereo microscopes can deliver a useful magnification up to 100×, comparable to a 10× objective and 10× ...
A Wild M400 macroscope. A macroscope or photomacroscope in its camera-equipped version (in German: makroskop / photomakroskop) is a type of optical microscope developed and named by Swiss microscope manufacturers Wild Heerbrugg and later, after that company's merger with Leica in 1987, by Leica Microsystems of Germany, optimised for high quality macro photography and/or viewing using a single ...
The fluorescence light emitted from the lightsheet is then collected perpendicularly with a standard microscope objective and projected onto an imaging sensor (usually a CCD, electron-multiplying CCD or CMOS camera). In order to let enough space for the excitation optics/lightsheet an observation objective with high working distance is used.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
In certain applications, long-working-distance or long-focus microscopes [31] are beneficial. An item may need to be examined behind a window, or industrial subjects may be a hazard to the objective. Such optics resemble telescopes with close-focus capabilities. [32] [33] Measuring microscopes are used for precision measurement.
Spectral precision distance microscopy (SPDM) is a family of localizing techniques in fluorescence microscopy which gets around the problem of there being many sources by measuring just a few sources at a time, so that each source is "optically isolated" from the others (i.e., separated by more than the microscope's resolution, typically ~200 ...
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