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Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
Örjan Thomas Ouchterlony (January 14, 1914, Stockholm – September 25, 2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [1] [2] He was trained at Karolinska Institute, where his received his medical doctorate.
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
A more specific test such as CIEP will follow with samples that are identified as positive. [6] The six main antigens used in immunological laboratories for detection are Ro, La, Sm, RNP, Scl-70 and Jo1, [7] which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting.
Counter-immunoelectrophoresis and its modification. In comparison to other conventional methods of diagnosis e.g. for viral infection testing, counter-immunoelectrophoresis is a highly specific, simple, and speedy method that does not require sophisticated, expensive tools, input materials, or long-term capacity building.
Örjan Ouchterlony (1914–2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [4] [5] Ouchterlony, Swedish noble family of Scottish origin. Descents of John Ouchterlony († 1778), from Dundee in Scotland.
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Ouchterlony double immunodiffusion; P. Plaque reduction neutralization test; W. Western blot This page was last edited on 5 May 2020, at 13:30 (UTC). Text ...