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  2. Single-cell analysis - Wikipedia

    en.wikipedia.org/wiki/Single-cell_analysis

    This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cellcell interactions at the level of an individual cell, as opposed to more ...

  3. Cell isolation - Wikipedia

    en.wikipedia.org/wiki/Cell_isolation

    Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.

  4. Single-cell sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-cell_sequencing

    Single-nucleotide polymorphisms (SNPs), which are a big part of genetic variation in the human genome, and copy number variation (CNV), pose problems in single cell sequencing, as well as the limited amount of DNA extracted from a single cell. Due to scant amounts of DNA, accurate analysis of DNA poses problems even after amplification since ...

  5. Protein metabolism - Wikipedia

    en.wikipedia.org/wiki/Protein_metabolism

    Protein anabolism is the process by which proteins are formed from amino acids. It relies on five processes: amino acid synthesis, transcription, translation, post translational modifications, and protein folding. Proteins are made from amino acids. In humans, some amino acids can be synthesized using already existing intermediates. These amino ...

  6. Transcriptome - Wikipedia

    en.wikipedia.org/wiki/Transcriptome

    Transcription can also be studied at the level of individual cells by single-cell transcriptomics. Single-cell RNA sequencing (scRNA-seq) is a recently developed technique that allows the analysis of the transcriptome of single cells, including bacteria. [25] With single-cell transcriptomics, subpopulations of cell types that constitute the ...

  7. Single-cell transcriptomics - Wikipedia

    en.wikipedia.org/wiki/Single-cell_transcriptomics

    Detecting differences in gene expression level between two populations is used both single-cell and bulk transcriptomic data. Specialised methods have been designed for single-cell data that considers single cell features such as technical dropouts and shape of the distribution e.g. Bimodal vs. unimodal. [23]

  8. Methionine synthase - Wikipedia

    en.wikipedia.org/wiki/Methionine_synthase

    In humans it is encoded by the MTR gene (5-methyltetrahydrofolate-homocysteine methyltransferase). [ 5 ] [ 6 ] Methionine synthase forms part of the S-adenosylmethionine (SAMe) biosynthesis and regeneration cycle, [ 7 ] and is the enzyme responsible for linking the cycle to one-carbon metabolism via the folate cycle.

  9. Protein phosphatase 1 - Wikipedia

    en.wikipedia.org/wiki/Protein_phosphatase_1

    PP1 has been found to be important in the control of glycogen metabolism, muscle contraction, cell progression, neuronal activities, splicing of RNA, mitosis, [1] cell division, apoptosis, protein synthesis, and regulation of membrane receptors and channels. [2]

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