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TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...
Dithiobutylamine (DTBA) is a reducing agent intended as an alternative for DTT in biochemical uses. It was designed to be easily synthesized in non-racemic form, to have a lower pKa (allowing more effective reduction at neutral pH), and to have a low disulfide E°′ reduction potential. [1]
The thiol reagents are used in excess to drive the equilibrium to the right: + + The reductant tris(2-carboxyethyl)phosphine (TCEP) is useful, beside being odorless compared to β-ME and DTT, because it is selective, working at both alkaline and acidic conditions (unlike DTT), is more hydrophilic and more resistant to oxidation in air ...
Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol . DTT is redox reagent also known as Cleland's reagent , after W. Wallace Cleland . [ 2 ]
Cultrex BME is used as an attachment substrate to promote the expansion and maintenance of induced pluripotent stem cells and embryonic stem cells in the absence of feeder cells. [7] The extracellular matrix proteins that compose the basement membrane extract are needed to maintain stem cells in an undifferentiated state during prolonged cell ...
Replacing a valve stem. A valve stem is a self-contained valve that opens to admit gas to a chamber (such as air to inflate a tire), [1] and is then automatically closed and kept sealed by the pressure in the chamber, or a spring, [2] [3] or both, to prevent the gas from escaping.
The Dunlop valve, (also called a Woods valve, an English valve or a Blitz valve [1]) is a type of pneumatic valve stem in use—mostly on inner tubes of bicycles—in many countries, including Japan, [2] Korea, India, Pakistan, Sri Lanka, most European countries, and a number of developing countries.
In the 1980s, many U.S. production engine remanufacturers began reaming valve guides, rather than replacing them, as part of their remanufacturing process. They found that by reaming all the valve guides in a head to one standard size (typically 0.008 in. diametrically oversized), and installing remanufactured engine valves having stems that are also oversized, a typical engine head can be ...