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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
The variable UV-Vis absorbance detector's optical bench is showing how the flow cell is positioned after the optical system, including the monochromator, which typically has a physical slit and a moving grating, so it is illuminated by a selected wavelength, reaching a photo-diode.
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
[17]: 18 Its designation as a "UV–Vis" spectrophotometer indicates its ability to measure light in both the visible and ultraviolet spectra. [ 29 ] The DU was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.
An XPS spectrometer. A spectrometer (/ s p ɛ k ˈ t r ɒ m ɪ t ər /) is a scientific instrument used to separate and measure spectral components of a physical phenomenon. Spectrometer is a broad term often used to describe instruments that measure a continuous variable of a phenomenon where the spectral components are somehow mixed.
The Spectronic 20 is a brand of single-beam spectrophotometer, designed to operate in the visible spectrum [1] across a wavelength range of 340 nm to 950 nm, with a spectral bandpass of 20 nm. [ 2 ] [ 3 ] It is designed for quantitative absorption measurement at single wavelengths. [ 1 ]
It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.
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