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DPPH has two major applications, both in laboratory research: one is a monitor of chemical reactions involving radicals, most notably it is a common antioxidant assay, [1] and another is a standard of the position and intensity of electron paramagnetic resonance signals.
Most commonly, antioxidant capacity is measured using the ABTS Decolorization Assay. Other antioxidant capacity assays which use Trolox as a standard include the diphenylpicrylhydrazyl ( DPPH ), oxygen radical absorbance capacity ( ORAC ) and ferric reducing ability of plasma ( FRAP ) assays.
Ferric reducing ability of plasma (FRAP, also Ferric ion reducing antioxidant power) is an antioxidant capacity assay that uses Trolox as a standard. [1] The FRAP assay was first performed by Iris Benzie and J. J. Strain of the Human Nutrition Research Group at the University of Ulster, Coleraine.
For example, polyphenol compounds, which widely exist in fruit, can quench free radicals inside human body, thus prevent oxidative damage by free radicals. The antioxidant potency of plant extract or food product has been measured by ABTS assay. One example with detailed method is the antioxidant activity analysis of Hibiscus products. [7]
Federal Rules of Appellate Procedure; Ferric Reducing Ability of Plasma, also Ferric ion reducing antioxidant power, a simple assay of antioxidant content in foods; Fluorescence recovery after photobleaching, an experimental technique in cell biology; Fluoride-resistant acid phosphatase
The Folin–Ciocâlteu reagent (FCR) or Folin's phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE). [1] It is named after Otto Folin, Vintilă Ciocâlteu, and Willey ...
Other antioxidant capacity assays which use Trolox as a standard include the diphenylpicrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), [26] ferric reducing ability of plasma (FRAP) [27] assays or inhibition of copper-catalyzed in vitro human low-density lipoprotein oxidation. [28]
3,5-Dimethylpiperidines are chemical compounds with the formula C 5 H 8 (CH 3) 2 NH. Two diastereomers exist: the achiral R,S isomer and the chiral R,R/S,S enantiomeric pair. 3,5-Dimethylpiperidine is a precursor to tibric acid.
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