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Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). [1] [2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of ...
RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)
The ristocetin-induced platelet aggregation (RIPA) is an ex vivo assay for live platelet function. It measures platelet aggregation with the help of von Willebrand factor (vWF) and exogenous antibiotic ristocetin added in a graded fashion. [ 1 ]
Radioimmunoprecipitation assay buffer (RIPA buffer), a radioimmunoprecipitation assay buffer; Regulation of Investigatory Powers Act 2000, a UK law governing interception of communications (information technology) Ristocetin-induced platelet aggregation, an assay for von Willebrand disease
Karnovsky's fixative solution is often modified for specialized applications. [2] [3] For example, 2% paraformaldehyde, 2.5% glutaraldehyde in 0.1 Molar sodium phosphate buffer (pH 7.4) has been used to study the ultrastructure of renal pelvis fragments. [3]
7099 21898 Ensembl ENSG00000136869 ENSMUSG00000039005 UniProt O00206 Q9QUK6 RefSeq (mRNA) NM_138557 NM_003266 NM_138554 NM_138556 NM_021297 RefSeq (protein) NP_003257 NP_612564 NP_612567 NP_067272 Location (UCSC) Chr 9: 117.7 – 117.72 Mb Chr 4: 66.75 – 66.85 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Toll-like receptor 4 (TLR4), also designated as CD284 (cluster of ...
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Tyrode's solution is a solution that is roughly isotonic with interstitial fluid and used in physiological experiments and tissue culture. It resembles lactated Ringer's solution, but contains magnesium, a sugar (usually glucose) as an energy source and uses bicarbonate and phosphate as a buffer instead of lactate.