Search results
Results from the WOW.Com Content Network
Using a mutated pseudo-wild-type protein specifically mutated to prevent precipitation at high pH, the salt bridge’s contribution to the overall free energy of the folded protein state can be determined by performing a point-mutation, altering and, consequently, breaking the salt bridge. For example, a salt bridge was identified to exist in ...
In electrochemistry, a salt bridge or ion bridge is an essential laboratory device discovered over 100 years ago. [ 1 ] It contains an electrolyte solution, typically an inert solution, used to connect the oxidation and reduction half-cells of a galvanic cell (voltaic cell), a type of electrochemical cell .
Example salt bridge network in 2-deoxyribose-5-phosphate aldolase ( ). Interactions are shown as cyan dashed lines. Polar residues are colored green. Polar amino acids aspartate (D), glutamate (E), lysine (K), and arginine (R), are shown here. Salt bridges within TIM barrel pores are thought to contribute to the overall stability of the fold.
A chaotrope denatures biomolecules by disrupting the shell of hydration around them. This allows positively charged ions to form a salt bridge between the negatively charged silica and the negatively charged DNA backbone in high salt concentration. The DNA can then be washed with high salt and ethanol, and ultimately eluted with low salt.
Other mechanisms include minimization of solvent accessibility of acidic residues or binding of metal cofactors. In a specialized case of acid stability, the NAPase protein from Nocardiopsis alba was shown to have relocated acid-sensitive salt bridges away from regions that play an important role in the unfolding process. In this case of ...
This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms.It is intended as introductory material for novices; for more specific and technical definitions from sub-disciplines and related fields, see Glossary of cell biology, Glossary of genetics, Glossary of evolutionary biology, Glossary of ecology ...
In electrochemistry, cell notation or cell representation is a shorthand method of expressing a reaction in an electrochemical cell.. In cell notation, the two half-cells are described by writing the formula of each individual chemical species involved in the redox reaction across the cell, with all other common ions and inert substances being ignored.
An example of a galvanic cell consists of two different metals, each immersed in separate beakers containing their respective metal ions in solution that are connected by a salt bridge or separated by a porous membrane. [1] Volta was the inventor of the voltaic pile, the first electrical battery.