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Reducing sugars. Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper (II) sulfate pentahydrate. [ 1 ] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing ...
It reacts with a reducing sugar to form 3-amino-5-nitrosalicylic acid, which can be measured by spectrophotometry to determine the amount of reducing sugar that was present. [8] Some sugars, such as sucrose, do not react with any of the reducing-sugar test solutions. However, a non-reducing sugar can be hydrolyzed using dilute hydrochloric acid ...
Monosaccharides. In organic chemistry, Fehling's solution is a chemical reagent used to differentiate between water-soluble carbohydrate and ketone (>C=O) functional groups, and as a test for reducing sugars and non-reducing sugars, supplementary to the Tollens' reagent test. The test was developed by German chemist Hermann von Fehling in 1849.
A disaccharide (also called a double sugar or biose) [ 1 ] is the sugar formed when two monosaccharides are joined by glycosidic linkage. [ 2 ] Like monosaccharides, disaccharides are simple sugars soluble in water. Three common examples are sucrose, lactose, and maltose. Disaccharides are one of the four chemical groupings of carbohydrates ...
In organic chemistry, a ketose is a monosaccharide containing one ketone (>C=O) group per molecule. [1][2] The simplest ketose is dihydroxyacetone ((CH2OH)2C=O), which has only three carbon atoms. It is the only ketose with no optical activity. All monosaccharide ketoses are reducing sugars, because they can tautomerize into aldoses via an ...
α-Glucosidase hydrolyzes terminal non-reducing (1→4)-linked α-glucose residues to release a single α-glucose molecule. [ 10 ] α-Glucosidase is a carbohydrate-hydrolase that releases α-glucose as opposed to β-glucose. β-Glucose residues can be released by glucoamylase, a functionally similar enzyme.
Oligosaccharide. An oligosaccharide (/ ˌɒlɪɡoʊˈsækəˌraɪd /; [1] from Ancient Greek ὀλίγος (olígos) 'few' and σάκχαρ (sákkhar) 'sugar') is a saccharide polymer containing a small number (typically three to ten [2][3][4][5]) of monosaccharides (simple sugars). Oligosaccharides can have many functions including cell ...
Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...