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De novo sequence assemblers are a type of program that assembles short nucleotide sequences into longer ones without the use of a reference genome.These are most commonly used in bioinformatic studies to assemble genomes or transcriptomes.
For example, a custom in-house application might use DDE to open a Microsoft Excel spreadsheet and fill it with data, by opening a DDE conversation with Excel and sending it DDE commands. This feature has mainly been superseded by the Component Object Model suite of technologies. Microsoft has not used DDE internally since the release of ...
It offers a solution to map NGS short reads with a moderate distance (up to 30% sequence divergence) from reference genomes. It poses no restrictions on the size of the reference, which, combined with its high sensitivity, makes the Variant Toolkit well-suited for targeted sequencing projects and diagnostics.
In its simplest form, a fluorescently labelled probe hybridizes to its complementary sequence adjacent to the primed template. DNA ligase is then added to join the dye-labelled probe to the primer. Non-ligated probes are washed away, followed by fluorescence imaging to determine the identity of the ligated probe.
Dynamic Data Exchange, a Microsoft Windows and OS/2 inter-application data communication protocol; Escort destroyer, a US Navy classification used between 1945 and 1962; D.De., an abbreviation used for the United States District Court for the District of Delaware; Deepin Desktop Environment, a Desktop Environment used by several Linux Distributions
In two-base encoding, each unique pair of bases on the 3' end of the probe is assigned one out of four possible colors. For example, "AA" is assigned to blue, "AC" is assigned to green, and so on for all 16 unique pairs. During sequencing, each base in the template is sequenced twice, and the resulting data are decoded according to this scheme.
Broader application benefited from pairwise end sequencing, known colloquially as double-barrel shotgun sequencing.As sequencing projects began to take on longer and more complicated DNA sequences, multiple groups began to realize that useful information could be obtained by sequencing both ends of a fragment of DNA.
An important part of the design of variant calling methods using NGS data is the DNA sequence used as a reference to which the NGS reads are aligned. In human genetics studies, high quality references are available, from sources such as the HapMap project , [ 10 ] which can substantially improve the accuracy of the variant calls made by variant ...