Search results
Results from the WOW.Com Content Network
Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [ 2 ] large proteins , [ 3 ] small nucleotides , [ 4 ] and amino acids .
Electrophoresis is the basis for analytical techniques used in biochemistry for separating particles, molecules, or ions by size, charge, or binding affinity either freely or through a supportive medium using a one-directional flow of electrical charge. [10] It is used extensively in DNA, RNA and protein analysis. [11]
In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances [3] with a negative charge at higher pH levels. The ...
The net charge of an ion is not zero because its total number of electrons is unequal to its total number of protons. A cation is a positively charged ion with fewer electrons than protons [2] (e.g. K + (potassium ion)) while an anion is a negatively charged ion with more electrons than protons. [3] (e.g. Cl-(chloride ion) and OH-(hydroxide ion)).
The anion gap is the quantity difference between cations (positively charged ions) and anions (negatively charged ions) in serum, plasma, or urine. The magnitude of this difference (i.e., "gap") in the serum is calculated to identify metabolic acidosis. If the gap is greater than normal, then high anion gap metabolic acidosis is diagnosed.
Under the influence of an electrical potential difference, the negatively charged ions (e.g., chloride) in the dilute stream migrate toward the positively charged anode. These ions pass through the positively charged anion-exchange membrane, but are prevented from further migration toward the anode by the negatively charged cation-exchange ...
So for example, for a negatively charged particle, <, and if the positively charged ions diffuse faster than the negatively charged ones, then this term will push particles down a salt gradient, but if it is the negatively charged ions that diffuse faster, then this term pushes the particles up the salt gradient.
On the contrary, if the species has gained one or several additional electrons, it becomes negatively charged and is called a negative ion, or anion. Individual free electrons and ions in a plasma have very short lives typically inferior to the microsecond, as ionization and recombination, excitation and relaxation are collective continuous ...