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The multiple crystals on the right were grown from a sugar cube, while the one on the left was grown from a single seed taken from the one on the right. Red dye was added to the sugar solution before growing the large crystal, but was insoluble with the sugar in its solid state, and all but small traces of the dye was forced to precipitate out ...
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.
Crystallographic image processing (CIP) is traditionally understood as being a set of key steps in the determination of the atomic structure of crystalline matter from high-resolution electron microscopy images obtained in a transmission electron microscope that is run in the parallel illumination mode.
At first resolution was poor, with in 1956 James Menter publishing the first electron microscope images showing the lattice structure of a material at 1.2nm resolution. [38] In 1968 Aaron Klug and David DeRosier used electron microscopy to visualise the structure of the tail of bacteriophage T4, a common virus, a key step in the use of ...
Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). [1] These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses .
Image SXM is an image analysis software specialized in scanning microscope images. It is based on the public domain software NIH Image (now ImageJ from the National Institutes of Health ) and extended to handle scanning microscope images, especially of the SxM formats (SAM, SCM, SEM, SFM, SLM, SNOM, SPM, STM), hence its name.
The basic process by which imaging particle analysis is carried out is as follows: A digital camera captures an image of the field of view in the optical system.; A gray scale thresholding process is used to perform image segmentation, segregating out the particles from the background, creating a binary image of each particle.
Under ideal conditions, this produces an "hourglass" shape of this point source in the third (axial) dimension. This shape is called the point spread function (PSF) of the microscope imaging system. Since any fluorescence image is made up of a large number of such small fluorescent light sources, the image is said to be "convolved by the point ...