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The method is based on digesting a mixture of PCR amplified variants of a single gene using one or more restriction enzymes and detecting the size of each of the individual resulting terminal fragments using a DNA sequencer. The result is a graph image where the x-axis represents the sizes of the fragment and the y-axis represents their ...
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
Community fingerprinting is a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community. Rather than directly identifying or counting individual cells in an environmental sample, these techniques show how many variants of a gene are present. In general, it is assumed that each different gene ...
Because molecular diagnostics methods can detect sensitive markers, these tests are less intrusive than a traditional biopsy. For example, because cell-free nucleic acids exist in human plasma , a simple blood sample can be enough to sample genetic information from tumours, transplants or an unborn fetus.
Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
Example: molecular dynamics of a glucose-tolerant β-Glucosidase [32] Molecular dynamics (MD) is a computational method for simulating interactions between molecules and their atoms during a given period of time. [33] This method allows the observation of the behavior of molecules and their interactions, considering the system as a whole.