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  2. Phage display - Wikipedia

    en.wikipedia.org/wiki/Phage_display

    Phage display is also a widely used method for in vitro protein evolution (also called protein engineering). As such, phage display is a useful tool in drug discovery. It is used for finding new ligands (enzyme inhibitors, receptor agonists and antagonists) to target proteins.

  3. Methods to investigate protein–protein interactions - Wikipedia

    en.wikipedia.org/wiki/Methods_to_investigate...

    Phage display is used for the high-throughput screening of protein interactions. In-vivo crosslinking of protein complexes using photo-reactive amino acid analogs was introduced in 2005 by researchers from the Max Planck Institute [ 5 ] In this method, cells are grown with photoreactive diazirine analogs to leucine and methionine , which are ...

  4. Protein engineering - Wikipedia

    en.wikipedia.org/wiki/Protein_engineering

    Phage display methods are one option for screening proteins. This method involves the fusion of genes encoding the variant polypeptides with phage coat protein genes. Protein variants expressed on phage surfaces are selected by binding with immobilized targets in vitro.

  5. Directed evolution - Wikipedia

    en.wikipedia.org/wiki/Directed_evolution

    The red symbols indicate functional variants, the pale symbols indicate variants with reduced function. Directed evolution ( DE ) is a method used in protein engineering that mimics the process of natural selection to steer proteins or nucleic acids toward a user-defined goal. [ 1 ]

  6. Two-hybrid screening - Wikipedia

    en.wikipedia.org/wiki/Two-hybrid_screening

    The resulting phage particles that are produced contain the single-stranded phagemids and are used to infect XL-1 Blue cells. [2] The double-stranded phagemids are subsequently collected from these XL-1 Blue cells, essentially reversing the process used to produce the original library phage.

  7. Bacterial display - Wikipedia

    en.wikipedia.org/wiki/Bacterial_display

    Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning). This protein engineering technique allows us to link the ...

  8. John McCafferty - Wikipedia

    en.wikipedia.org/wiki/John_McCafferty

    John McCafferty is a British scientist, one of the founders of Cambridge Antibody Technology alongside Sir Gregory Winter and David Chiswell. He is well known as one of the inventors of scFv antibody fragment phage display, [1] a technology that revolutionised the monoclonal antibody drug discovery.

  9. Zinc finger chimera - Wikipedia

    en.wikipedia.org/wiki/Zinc_finger_chimera

    In order to determine the most appropriate sequence of amino acids in the alpha-helix of a zinc finger for binding to a given DNA sequence, a technique involving phage display may be employed. By altering the genome of selected bacteriophage, it is possible to create a phage that will display a ZFP as part of its protein coat.

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