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A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. [1] Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical ...
The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison. In 1972 Good published a second list with three more buffers, and five more were added in 1980.
A category for buffering agents and buffer solutions made with them. Pages in category "Buffer solutions" The following 39 pages are in this category, out of 39 total
[72] [73] [74] The isoelectric point of a given molecule is a function of its pK values, so different molecules have different isoelectric points. This permits a technique called isoelectric focusing, [75] which is used for separation of proteins by 2-D gel polyacrylamide gel electrophoresis. Buffer solutions also play a key role in analytical ...
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
TES is used to make buffer solutions. It has a pK a value of 7.550 (I=0, 25°C). [1] It is one of the Good's buffers and can be used to make buffer solutions in the pH range 6.8–8.2. It is one of the components of Test yolk buffer medium used for refrigeration and transport of semen.
It has pK a value of 6.15 at 20 °C. The pH (and pK a at ionic strength I≠0) of the buffer solution changes with concentration and temperature, and this effect may be predicted using online calculators. [2] MES is highly soluble in water. The melting point is approx. 300 °C. MES was developed as one of Good's buffers in the 1960s.
Total ionic strength adjustment buffer (TISAB) is a buffer solution which increases the ionic strength of a solution to a relatively high level. This is important for potentiometric measurements, including ion selective electrodes , because they measure the activity of the analyte rather than its concentration.