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Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel.
TPCK-treated trypsin is used to improve infection yield in laboratory tissue culture of some wild virus isolates that are not well-adapted to growth in vitro, such as some low-pathogenic avian influenza strains or fresh clinical isolates of SARS-CoV-2. The trypsin performs the maturation cleavage of the viral envelope proteins efficiently.
Trypsin is an enzyme in the first section of the small intestine that starts the digestion of protein molecules by cutting long chains of amino acids into smaller pieces. It is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins.
A diaphonized mirror dory.The bones are dyed red and the cartilage is dyed blue. Diaphonized veiled chameleon. Field Museum of Natural History, Chicago.. Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the animal transparent by bathing it in trypsin, and then stains the bones and cartilage ...
Blue is the range of Felinae (excluding the domestic cat), green is the range of Pantherinae. Felidae is a family of mammals in the order Carnivora, colloquially referred to as cats. A member of this family is called a felid. [1] [2] The term "cat" refers both to felids in general and specifically to domestic cats.
Fever coat is an effect known in domestic cats, where a pregnant female cat has a fever or is stressed, causing her unborn kittens' fur to develop a silver-type color (silver-grey, cream, or reddish) rather than what the kitten's genetics would normally cause. After birth, over some weeks the silver fur is replaced naturally by fur colors ...
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. [1] Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2] It provides a source of amino acids for the growing bacteria.
The brain of the domesticated cat is about five centimetres (2.0 in) long and weighs 25–30 g (0.88–1.06 oz). [1] [2] If a typical cat is taken to be 60 cm (24 in) long with a weight of 3.3 kg (7.3 lb), then the brain would be at 0.91% [3] of its total body mass, compared to 2.33% [3] of total body mass in the average human.
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