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Proteus mirabilis appears as Gram-negative rods after Gram staining under bright-field microscopy with 1000 times magnification. Proteus mirabilis is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans.
The term Proteus signifies changeability of form, as personified in the Homeric poems in Proteus, "the old man of the sea", who tends the sealflocks of Poseidon and has the gift of endless transformation. The first use of the term “Proteus” in bacteriological nomenclature was made by Hauser (1885), who described under this term three types ...
Xylose lysine deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. [ 1 ] [ 2 ] The agar was developed by Welton Taylor in 1965. [ 3 ]
A third technique is using sterile glass beads to plate out cells. In this technique, cells are grown in a liquid culture, in which a small volume is pipetted on the agar plate and then spread out with the beads. Replica plating is another technique used to plate out cells on agar plates. These four techniques are the most common, but others ...
The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group.A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours.
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar ; however, the agar is supplemented with the surfactant, Tergitol 4 , which causes inhibition of Proteus spp. and other non-Salmonellae. [ 1 ]
Agar plates are used as a canvas, while pigmented or fluorescent bacteria and yeasts represent paint. In order to preserve a piece of microbial art after a sufficient incubation, the microbe culture is sealed with epoxy. [2] Microbe species can be artistically chosen for their natural colours to form a palette.