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a clear solution of blood plasma in the upper phase (which can be separated into its own fractions, see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets in the middle, and; erythrocytes (red blood cells) at the bottom of the centrifuge tube.
The process of blood fractionation involves separation of blood into its main components. Blood fractionation refers generally to the process of separation using a centrifuge (centrifugation), after which three major blood components can be visualized: plasma, buffy coat and erythrocytes (blood cells). These separated components can be analyzed ...
After centrifugation, one can distinguish a layer of clear fluid (the plasma), a layer of red fluid containing erythrocytes, and a thin layer in between.Composing less than 1% of the total volume of the blood sample, the buffy coat (so-called because it is usually buff in hue), contains most of the leukocytes and thrombocytes.
These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, [3] which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and ...
In erythrocytapheresis, centrifugation is the most commonly used red blood cell fractionation method. This is because the hematocrit, or the percentage of blood volume taken up by red blood cells, is present in the highest percentage of all blood cell components in the solid portion of blood. Therefore, since erythrocytes have the highest ...
Blood compatibility testing is routinely performed before a blood transfusion.The full compatibility testing process involves ABO and RhD (Rh factor) typing; screening for antibodies against other blood group systems; and crossmatching, which involves testing the recipient's blood plasma against the donor's red blood cells as a final check for incompatibility.
Typically, a 300 ml batch of blood is removed at a time and centrifuged to separate plasma from blood cells. Continuous flow centrifugation: Two venous lines are used. This method requires slightly less blood volume out of the body at any one time, as it is able to continuously spin out plasma. Plasma filtration: Two venous lines are used.
A cytocentrifuge, sometimes referred to as a cytospin, [1] is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined. [2] Cytocentrifuges are used in various areas of the clinical laboratory, such as cytopathology, hematology and microbiology, as well as in ...