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Collagen is the main structural protein found in the body's various connective tissues (skin, bones, tendons, etc.) where it contributes to much of their strength and elasticity. Collagen loss occurs naturally as a part of aging, but can also be influenced by environmental factors such as exposure to ultraviolet light, tobacco , and excessive ...
See protein electrophoresis for more information on the different globulins. Nearly all enzymes with major metabolic functions are globular in shape, as well as many signal transduction proteins. Albumins are also globular proteins, although, unlike all of the other globular proteins, they are completely soluble in water. They are not soluble ...
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
For example, basic residues on a protein can have electrostatic interactions with acidic residues on another protein. However, solvation by ions in an electrolytic solution or water will decrease protein–protein attractive forces. Therefore, to precipitate or induce accumulation of proteins, the hydration layer around the protein should be ...
Fibrous proteins are structural or storage proteins that are typically inert and water-insoluble. A fibrous protein occurs as an aggregate due to hydrophobic side chains that protrude from the molecule. A fibrous protein's peptide sequence often has limited residues with repeats; these can form unusual secondary structures, such as a collagen ...
Collagen is a vital protein in skin, hair, nails, and other tissues. Its production decreases with age and factors like sun damage and smoking. Collagen supplements, derived from sources like fish and cattle, are marketed to improve skin, hair, and nails. Studies show some skin benefits, but these supplements often contain other beneficial ...
A classic example is when water molecules arrange around a metal ion. If the metal ion is a cation, the electronegative oxygen atom of the water molecule would be attracted electrostatically to the positive charge on the metal ion. The result is a solvation shell of water molecules that surround the ion.
By using the Bradford protein assay, one can avoid all of these complications by simply mixing the protein samples with the Coomassie brilliant blue G-250 dye (Bradford reagent) and measuring their absorbances at 595 nm, which is in the visible range [8] and may be accurately measured by the use of a mobile smartphone camera. [9]