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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers . HEPES is widely used in cell culture , largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration ) when compared ...
The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison.
HEPPS (EPPS) is a buffering agent used in biology and biochemistry.The pKa of HEPPS is 8.00. It is ones of Good's buffers. [1]Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice.
Buffer capacity falls to 33% of the maximum value at pH = pK a ± 1, to 10% at pH = pK a ± 1.5 and to 1% at pH = pK a ± 2. For this reason the most useful range is approximately pK a ± 1. When choosing a buffer for use at a specific pH, it should have a pK a value as close as possible to that pH. [2]
After the Eastern Band of Cherokee Indians approved the adult use of recreational marijuana in September 2023, the first legal cannabis dispensary in North Carolina is set to open on ECBI land ...
Currently the best source of light for cannabis remains to be metal halide or ceramic metal halide in the range of 3-4000k. Cannabis plants also require both dark and light photoperiods, so the lights need a timer to switch them on and off at set intervals. The optimum photoperiod depends on each plant (some prefer long days and short nights ...
The flowers of Cannabis sativa plants are most often either male or female, but, only plants displaying female pistils can be or turn hermaphrodite. Males can never become hermaphrodites. [ 3 ] It is a short-day flowering plant, with staminate (male) plants usually taller and less robust than pistillate (female or male) plants.
Cannabis plants produce a unique family of terpeno-phenolic compounds called cannabinoids, some of which produce the "high" which may be experienced from consuming marijuana. There are 483 identifiable chemical constituents known to exist in the cannabis plant, [53] and at least 85 different cannabinoids have been isolated from the plant. [54]