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A set of standard 75 by 25 mm microscope slides. The white area can be written on to label the slide. A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope.
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image.
A small piece of absorbent paper may be touched to the edge of the cover slip to remove the excess water, until the cover glass affixes to the slide. The cover slip rocks on the specimen or is clearly not laying flat – either the specimen or something in the sample (a grain of sand, for example) is preventing the cover slip from coming down ...
In this technique, the specimen is sawed to ~1 mm thick, glued to a microscope slide, and ground or sawed (e.g., by microtome) to a thickness (x) approaching 30 μm. [11] [12] A cover slip is glued onto the exposed surface.
A number of slides will usually be prepared from different levels throughout the block. After this the thin section mounted slide is stained and a protective cover slip is mounted on it. For common stains, an automatic process is normally used; but rarely used stains are often done by hand. [1]
Optical microscope. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
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