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A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing. This ...
In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
Optical microscope. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
A hemocytometer. The two semi-reflective rectangles are the counting chambers. The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [1] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a ...
Interferometric scattering microscopy. Typical iSCAT configurations where either the reflected light from the cover-slip (a, c) or the transmitted light through the sample (b, d) is used as a reference field. The signal can be acquired with a camera in wide-field operation (a, b) or by point detection in confocal arrangement (c, d).
To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1]
Total internal reflection fluorescence microscope. A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is ...
The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion. Some microscopes also use other index-matching materials besides oil; see water immersion objective and solid immersion lens.
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