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Confocal microscopy is the microscopic procedure of choice for examining neuron structures as it produces sharp images with improved resolution and decreased signal-to-noise ratio. The specific way this microscopy works allows one to look at one confocal plane at a time, which is optimal when viewing neuronal structures.
Digital reconstruction or tracing of neuron morphology is a fundamental task in computational neuroscience. [1] [2] [3] It is also critical for mapping neuronal circuits based on advanced microscope images, usually based on light microscopy (e.g. laser scanning microscopy, bright field imaging) or electron microscopy or other methods.
The cells in nervous tissue are densely packed, and little information on their structures and interconnections can be obtained if all the cells are stained. Furthermore, the thin filamentary extensions of neural cells, including the axon and the dendrites of neurons, are too slender and transparent to be seen with normal staining techniques.
Images (3D, 4D) Various No Neuroelectro.org Electrophysiology of neurons Human, others Neuron Electrophysiological properties and data Healthy No [33] Neuromorpho.org 3D models of real neurons Human, rat, mouse, monkey, others Neuron Images and 3D data Healthy No [34] Neuromorphometrics.com: Manually labeled MRI Brain Scans Human Macroscopic
Nissl bodies occur in the somata and dendrites of neurons, though not in the axon or axon hillock. [6] They vary in size, shape, and intracellular location; they are most conspicuous in the motor neurons of the spinal cord and brainstem, where they appear as large, blocky assemblies. [5]