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A Gram stain of mixed Staphylococcus aureus (S. aureus ATCC 25923, gram-positive cocci, in purple) and Escherichia coli (E. coli ATCC 11775, gram-negative bacilli, in red), the most common Gram stain reference bacteria. Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups ...
It is also used to stain Wolbachia cells in host tissue. [3] Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens. Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta.
Staining Technique Preparation Application Result 1. Simple (Monochrome) Smear stain with single dye . e.g. Methylene blue, Safranin°≤×←→ etc. Used to highlight microbes and illustrate cellular shapes and arrangements Organisms are stained in the color of applied stain 2. Negative (Relief) Smear mixed with Nigrosin and spread into thin film
Thin smears are similar to usual blood films and allow species identification, because the parasite's appearance is best preserved in this preparation. Thick smears allow the microscopist to screen a larger volume of blood and are about eleven times more sensitive than the thin film, so picking up low levels of infection is easier on the thick ...
This diluted bacteria sample is commonly referred to as a smear after it is placed on a slide. After a smear has dried at room temperature, the slide is gripped by tongs or a clothespin and passed through the flame of a Bunsen burner several times to heat-kill and adhere the organism to the slide.
Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates .
When looking at the smears for TB, it is stained using an acid-fast stain. These acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. These acids resist staining by ordinary methods such as a Gram stain. [9] It can also be used to stain a few other bacteria, such as Nocardia.
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.