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A spore print is made by placing the spore-producing surface flat on a sheet of dark and white paper or on a sheet of clear, stiff plastic, which facilitates moving the spore print to a darker or lighter surface for improved contrast; for example, it is easier to determine whether the spore print is pure white or, rather, very slightly pigmented.
The lattice light-sheet microscope has two modes of operation: In the dithered mode, the light sheet is rapidly scanned along the x axis and only one image is recorded per Z plane, at normal diffraction limited resolutions. [1] The second mode of operation is the structured illumination microscopy mode (SIM).
Glass chart. A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens.
In contrast to epifluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated perpendicularly to the direction of observation. For illumination, a laser light-sheet is used, i.e. a laser beam which is focused only in one direction (e.g. using a cylindrical lens). A second method ...
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A live-cell microscope. Live-cell microscopes are generally inverted. To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy.
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning.
The earliest reference to the use of conoscopy (i.e., observation in convergent light with a polarization microscope with a Bertrand lens) for evaluation of the optical properties of liquid crystalline phases (i.e., orientation of the optical axes) is in 1911 when it was used by Charles-Victor Mauguin to investigate the alignment of nematic and ...
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