Search results
Results from the WOW.Com Content Network
Lysis (/ ˈ l aɪ s ɪ s / LY-sis; from Greek λῠ́σῐς lýsis 'loosening') is the breaking down of the membrane of a cell, often by viral, enzymic, or osmotic (that is, "lytic" / ˈ l ɪ t ɪ k / LIT-ik) mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a lysate.
Many components of animal cells are recycled by transferring them inside or embedded in sections of membrane. For instance, in endocytosis (more specifically, macropinocytosis), a portion of the cell's plasma membrane pinches off to form vesicles that will eventually fuse with an organelle within the cell. Without active replenishment, the ...
Cytolysis, or osmotic lysis, occurs when a cell bursts due to an osmotic imbalance that has caused excess water to diffuse into the cell. Water can enter the cell by diffusion through the cell membrane or through selective membrane channels called aquaporins, which greatly facilitate the flow of water. [ 1 ]
Lysis: This step involves breaking open the cells to release the DNA. For example, in the case of bacterial cells, a solution of detergent and salt (such as SDS) can be used to disrupt the cell membrane and release the DNA. For plant and animal cells, mechanical or enzymatic methods are often used.
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
Plasmolysis can be reversed if the cell is placed in a hypotonic solution. Stomata close to help keep water in the plant so it does not dry out. Wax also keeps water in the plant. The equivalent process in animal cells is called crenation. The liquid content of the cell leaks out due to exosmosis.
CwlC is found in the mother cell wall and functions for the lysis of the mother cell wall. [6] CwlC does not have a signal sequence but participates in late sporulation and is present in the cell wall. [14] [15] It was found in B. subtilis that CwlC is able to hydrolyze both vegetative cell walls and spore peptidoglycan. [14]
Lysozyme activity increases with increasing temperatures, up to 60 degrees Celsius, with a pH range of 6.0-7.0. The salts present also affect lysozyme treatment, where some assert inhibitory effects, and others promote lysis via lysozyme treatment. Sodium chloride induces lysis, but at high concentrations, it is an active inhibitor of lysis.