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Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) amplification that relies on a natural PCR primer sequence at the polyadenylation site, also known as the poly(A) tail, and adds a second primer through the activity of murine leukemia virus reverse transcriptase. [1]
A trap primer (or trap seal primer) is a plumbing device or valve that adds water to traps.The water seals in traps are needed to prevent sewer gases from entering buildings, but because this water is exposed to the air, it is subject to evaporation over time in infrequently used floor drains, leading to the release of sewer gas into the environment.
A PCR primer is a short chain of single-stranded DNA, consisting of roughly twenty nucleotides complementary to the target sequence of DNA. During PCR, two primers will bind to opposite template strands of DNA. The two primers point towards one another, allowing only a specific region of DNA to be copied. [9]
Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase.The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases.
The structure of the repeating unit of PPP Space-filling model of a short section of PPP. Poly(p-phenylene) (PPP) is made of repeating p-phenylene units, which act as the precursor to a conducting polymer of the rigid-rod polymer family. The synthesis of PPP has proven challenging, but has been accomplished through excess polycondensation with ...
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
OLIGO Primer Analysis Software is a software for DNA primer design. [ 1 ] [ 2 ] The first paper describing this software was published in 1989. [ 3 ] The program is a real time PCR primer and probe search and analysis tool.
The Penning–Malmberg trap (PM trap), named after Frans Penning and John Malmberg, is an electromagnetic device used to confine large numbers of charged particles of a single sign of charge. Much interest in Penning–Malmberg (PM) traps arises from the fact that if the density of particles is large and the temperature is low, the gas will ...