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Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.
Today, most in situ hybridization procedures use fluorescent probes to detect DNA sequences, and the process is commonly referred to as FISH (fluorescence in situ hybridization). A...
Fluorescence in situ hybridization (FISH) is a technique pathologists use in genetic testing to help diagnose diseases caused by chromosomal differences. Healthcare providers can also use FISH to detect gene changes that they can target to treat cancer.
Fluorescence in situ hybridization (abbreviated FISH) is a laboratory technique used to detect and locate a specific DNA sequence on a chromosome. In this technique, the full set of chromosomes from an individual is affixed to a glass slide and then exposed to a “probe”—a small piece of purified DNA tagged with a fluorescent dye.
Fluorescence in situ hybridization provides genomic and transcriptomic information in the spatial cellular context. Thanks to its unique advantages, it has found applications in cell biological and genomic research as well as clinical diagnostics in preventive and reproductive medicine and oncology.
Fluorescence in situ hybridization (FISH) uses DNA fragments incorporated with fluorophore-coupled nucleotides as probes to examine the presence or absence of complementary sequences in fixed cells or tissues under a fluorescent microscope.
Fluorescence in situ hybridization (FISH) is a laboratory-based test that can reveal the genetic makeup of a cancer. Sanam Loghavi, M.D., explains how the results help with diagnosis, developing a treatment plan and even post-treatment surveillance.
What is Fluorescent in situ hybridization? The FISH method is based on the phenomenon of the denaturation and renaturation of DNA duplex. The DNA is a stable duplex, under normal conditions hydrogen bonding between two strands (two between adenine and thymine; and three between cytosine and guanine) makes it stable.
FISH is a technique that uses DNA probes (small molecules of DNA that are designed to hybridise with a particular section of the genome) to detect specific complementary sequences on a chromosome. It is undertaken in situ on chromosomes spread on a slide and visualised by microscopy.
This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments.