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CRISPR gene editing (CRISPR, pronounced / ˈ k r ɪ s p ə r / (crisper), refers to a clustered regularly interspaced short palindromic repeats") is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified.
Jo Zayner (formerly Josiah Zayner; alternatively Josie; born February 8, 1981) is a biohacker, artist, and scientist best known for their self-experimentation and work making hands-on genetic engineering accessible to a lay audience, including CRISPR.
Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.
CRISPR-Cas design tools are computer software platforms and bioinformatics tools used to facilitate the design of guide RNAs (gRNAs) ...
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Unlike traditional CRISPR-Cas9, which introduces double-strand breaks to edit genes, CRISPRa employs a modified, catalytically inactive Cas9 (dCas9) fused with transcriptional activators to target promoter or enhancer regions, thereby boosting gene transcription. This method allows for precise control of gene expression, making it a valuable ...
The CRISPR process, a 2013 breakthrough in biology, provides a way of controlling the basic genetic processes of life. In addition, the film documentary considers several relevant questions including, How will this new gene-editing ability change our relationship with nature ? and, What will this new gene-editing ability mean for human evolution ?