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  2. Sodium dodecyl sulfate - Wikipedia

    en.wikipedia.org/wiki/Sodium_dodecyl_sulfate

    Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS), sometimes written sodium laurilsulfate, is an organic compound with the formula CH 3 (CH 2) 11 OSO 3 Na and structure H 3 C−(CH 2) 11 −O−S(=O) 2 −O − Na +. It is an anionic surfactant used in many cleaning and hygiene products. This compound is the sodium salt of the 12 ...

  3. SDS-PAGE - Wikipedia

    en.wikipedia.org/wiki/SDS-PAGE

    [1] [2] The combined use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel eliminates the influence of structure and charge, and proteins are separated by differences in their size. At least up to 2012, the publication describing it was the most frequently cited paper by a single author, and the second ...

  4. Polyacrylamide gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Polyacrylamide_gel...

    Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight. At the pH at which gel electrophoresis is carried out the SDS molecules are negatively charged and bind to proteins in a set ratio, approximately one molecule of SDS for every 2 amino acids.

  5. SDS - Wikipedia

    en.wikipedia.org/wiki/SDS

    Safety data sheet, on chemicals; Sodium dodecyl sulfate, a synthetic organic compound SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; Shwachman–Diamond syndrome, a congenital disorder; Sudden death syndrome, a soybean disease

  6. Lysis buffer - Wikipedia

    en.wikipedia.org/wiki/Lysis_buffer

    Sodium dodecyl sulfate (SDS): an anionic detergent that denatures proteins by disrupting their secondary and tertiary structures, it solubilizes cellular membranes and aids in protein extraction. c. Tween-20: a nonionic detergent is milder compared to SDS and Triton X-100.

  7. Bradford protein assay - Wikipedia

    en.wikipedia.org/wiki/Bradford_protein_assay

    Sodium dodecyl sulfate (SDS), a common detergent, may be found in protein extracts because it is used to lyse cells by disrupting the membrane lipid bilayer and to denature proteins for SDS-PAGE. While other detergents interfere with the assay at high concentration, the interference caused by SDS is of two different modes, and each occurs at a ...

  8. Southwestern blot - Wikipedia

    en.wikipedia.org/wiki/Southwestern_blot

    First, proteins are prepared with a mixture that exposes them to the denaturing sodium dodecyl sulfate (SDS) agent. This exposure not only converts the proteins from a folded conformation to an unfolded conformation but also establishes uniform charge among them as well contributing to the ease of separation on a size basis using polyacrylamide ...

  9. Protein purification - Wikipedia

    en.wikipedia.org/wiki/Protein_purification

    A detergent such as sodium dodecyl sulfate (SDS) can be used to dissolve cell membranes and keep membrane proteins in solution during purification; however, because SDS causes denaturation, milder detergents such as Triton X-100 or CHAPS can be used to retain the protein's native conformation during complete purification.

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