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[222] [223] CRISPR diversity is used as an analysis target to discern phylogeny and diversity in bacteria, such as in xanthomonads by Martins et al., 2019. [224]: 552 Early detections of plant pathogens by molecular typing of the pathogen's CRISPRs can be used in agriculture as demonstrated by Shen et al., 2020. [224]: 553
Land area used for genetically modified crops by country (1996–2009), in millions of hectares. In 2011, the land area used was 160 million hectares, or 1.6 million square kilometers. [54] In the US, by 2014, 94% of the planted area of soybeans, 96% of cotton and 93% of corn were genetically modified varieties.
CRISPR gene editing is a revolutionary technology that allows for precise, targeted modifications to the DNA of living organisms. Developed from a natural defense mechanism found in bacteria, CRISPR-Cas9 is the most commonly used system, that allows "cutting" of DNA at specific locations and either delete, modify, or insert genetic material.
The IGI sustainable agriculture program and its Plant Genomics and Transformation Facility has developed CRISPR protocols for editing over 30 common crop species, [17] and has worked on developing applications including protecting the world's chocolate supply from cacao swollen shoot virus, [55] [56] removing toxic cyanide precursors in cassava ...
Genetically modified crops are genetically modified plants that are used in agriculture. The first crops provided are used for animal or human food and provide resistance to certain pests, diseases, environmental conditions, spoilage or chemical treatments (e.g. resistance to a herbicide). [16]
CRISPR [43] is the leading genetic engineering method. [44] In 2014, Esvelt and coworkers first suggested that CRISPR/Cas9 might be used to build gene drives. [5] In 2015, researchers reported successful engineering of CRISPR-based gene drives in Saccharomyces [45], Drosophila, [46] and mosquitoes.
A paper demonstrated that genome wide activation could be used to determine which proteins are involved in mediated resistance to a specific drug. [7] Another paper used genome wide activation of long, noncoding RNAs and observed that increasing the expression of certain long noncoding RNAs conferred resistance to the drug vemurafenib. [16]
The different generations of nucleases used for genome editing and the DNA repair pathways used to modify target DNA. Genome editing, or genome engineering, or gene editing, is a type of genetic engineering in which DNA is inserted, deleted, modified or replaced in the genome of a living organism.