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  2. Fluorescence in situ hybridization - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_in_situ...

    Multiplexed error-robust fluorescence in situ hybridization [24] is a highly multiplexed version of smFISH. It uses combinatorial labeling, followed by imaging, and then error-resistant encoding [25] to capture a high number of RNA molecules and spatial localization within the cell. The capture of a large number of RNA molecules enables ...

  3. Fluorescent in situ sequencing - Wikipedia

    en.wikipedia.org/wiki/Fluorescent_in_situ_sequencing

    The fluorescent tags are then cleaved and washed away, and the next cycle is initiated. Each rolony – corresponding to a single “parent” DNA or RNA molecule in the tissue – thus appears across a series of fluorescent images, as a localized “spot” with a sequence of colors corresponding to the nucleotide sequence of the parent molecule.

  4. Flow-FISH - Wikipedia

    en.wikipedia.org/wiki/Flow-FISH

    Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...

  5. Q-FISH - Wikipedia

    en.wikipedia.org/wiki/Q-FISH

    Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.

  6. Chromogenic in situ hybridization - Wikipedia

    en.wikipedia.org/wiki/Chromogenic_in_situ...

    Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. [ 1 ] [ 2 ] It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene ...

  7. Fluorescent tag - Wikipedia

    en.wikipedia.org/wiki/Fluorescent_tag

    Fluorescence in situ hybridization (FISH), is an example of a genetic labeling technique that utilizes probes that are specific for chromosomal sites along the length of a chromosome, also known as chromosome painting. Multiple fluorescent dyes that each have a distinct excitation and emission wavelength are bound to a probe which is then ...

  8. Food testing strips - Wikipedia

    en.wikipedia.org/wiki/Food_testing_strips

    One type of food testing strip is an assay enzyme reactant test, which requires the strip to be dipped into a blended mixture of food or test samples, distilled water and a reagent. Such tests are designed specifically to detect those strains of E.coli and Salmonella that are harmful to humans. A second type of food testing strip is a gram ...

  9. Fluorescence in the life sciences - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_in_the_life...

    A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...