Search results
Results from the WOW.Com Content Network
While some cell lines are cultured in suspension, the majority of commercially available mammalian cell lines are adherent. [ 2 ] [ 3 ] Suspension cell cultures must be agitated to maintain cells in suspension, and may require specialized equipment (e.g. magnetic stir plate, orbital shakers, incubators) and flasks (e.g. culture flasks, spinner ...
Cell samples can be taken from tissue explants or cell suspension cultures. Adherent cell cultures with an excess of nutrient-containing growth medium will continue to grow until they cover the available surface area. [3] Proteases like trypsin are most commonly used to break the adhesion from the cells to the flask. Alternatively, cell ...
Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.
For adherent cells, cell density is normally measured in terms of confluency, the percentage of the growth surface covered by cells. The cells will often have a known range of confluencies for optimal growth, for example a mammalian cell line like HeLa generally prefers confluencies between 10% and 100%, and subculture will normally try to keep ...
Schematic of cell adhesion. Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released ...
The transport of a solute in or out of the cell, for example, is difficult to study because the specialized cells in a multicellular organism behave differently. Cell suspension cultures such as tobacco BY-2 provide good model systems for these studies at the level of a single cell and its compartments because tobacco BY-2 cells behave very ...
Microcarrier cell culture, however, was the breakthrough required for cell culture to reach industrial and clinical significance. [2] Studies have shown that microcarrier suspensions, compared to multi-layer vessel culture, improve cell yield by 80-fold at only ten percent of Good Manufacturing Practice space, and only sixty percent of the ...
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]