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Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure. The addition of iodine, which binds to crystal violet and traps it in the cell
The smear is first treated with chloroform to remove fats . Smear applied with Alberts stain which contains cationic dyes such as toluidine blue and malachite green. Toluidine blue preferentially stains granules while malachite green stains cytoplasm. The granules show the typical monochromatism nature, this is used to demonstrate granules
Using aseptic technique, prepare and air dried heat fixed slide with the desired organism. Prepare a boiling water bath. Cover the slide with a piece of paper towel and place on staining rack over the water bath. Flood the paper towel on the slide with Malachite Green (primary stain). Steam the slide for 5 to 7 minutes (mordant).
Catchment zone in Nattai, Australia containing drinking water. Catchment hydrology is the study of hydrology in drainage basins. Catchments are areas of land where runoff collects to a specific zone. This movement is caused by water moving from areas of high energy to low energy due to the influence of gravity.
Food grading involves the inspection, assessment and sorting of various foods regarding quality, freshness, legal conformity and market value. [1] [2] Food grading is often done by hand, in which foods are assessed and sorted. [1] [2] Machinery is also used to grade foods, and may involve sorting products by size, shape and quality.
Ultra-high temperature processing (UHT), ultra-heat treatment, or ultra-pasteurization [1] is a food processing technology that sterilizes liquid food by heating it above 140 °C (284 °F) – the temperature required to kill bacterial endospores – for two to five seconds. [2]
To heat a meal, the bag is first torn open, and a sealed food pouch is placed inside. About 1 US fluid ounce (30 mL) of water is then added to the bag, using the line printed on the bag as a marker. The chemical reaction begins immediately, and takes about 12 to 15 minutes to heat a food pouch to about 60 °C (140 °F).
Food contact material pictogram (left) on a plastic food container in Hong Kong. Food contact materials or food contacting substances (FCS) [1] [2] are materials that are intended to be in contact with food. These can be things that are quite obvious like a glass or a can for soft drinks as well as machinery in a food factory or a coffee machine.