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Image Analysis. I am very confused about the way ImageJ incorporates the HSB color model into the color threshold tool (Image -> Adjust). In this tool, if the HSB model is selected, it is represented in a scale of H (0-225), S (0-225) and B (0-225), while it should be H (0-360), S (0-100), B (0-100). I want to select a H threshold of 80, what ...
I want to process an image stack such that every pixel with a color value below, say, 2000 is changed to a color value of 10000. What is the simplest way to do this? you can do in a manual way the following steps, I am using th dot_blot.jpg example image that you can find in File>Open Samples…
Hello everyone, I want to show my 3D image by the Thickness result with color. How to draw the color scale bar? Like this： I know the color scale can be seen in the histogram of thickness images. Can this scale bar be added in ImageJ? Or need to use other software to do this？But how to determine the value along the color gradient？ Any leads will be appreciate！ Mengying
The method in ImageJ is obvious enough when the color image converts using a standard method to a grayscale with linear monotonic increase with elevation. In my current case, however, the color scale defining topography changes from dark red to lighter yellow to darker green to lighter blue to dark blue, which makes the standard grayscale image ...
getPixel(x, y) Returns the raw value of the pixel at (x,y). Uses bilinear interpolation if 'x' or 'y' are not integers. Use getValue(x,y) to get calibrated pixel values from 8 and 16 bit images and intensity values from RGB images. Note that pixels in RGB images contain red, green and blue components that need to be extracted.
the gray value is just a unitless number from 0 to 255 for 8-bit-images (2^8 = 256) or from 0 to 65535 for 16-bit-images (2^16 = 65536) which gives the “brightness” - the higher the value the “whiter” the pixel. You can normalize an image to the [0,1]-range by converting it to 32-bit (Image - Type - 32bit) and dividing by 255 ...
Hi, For my graduate project I need help in image analysis I need to find an average RGB for each particle in an image ( Like if 20 particles are spread throughout a white sheet , I need to 20 RGB values ) Thing is that,Using Imagej I could find RGB of each pixel but not average RGB of a particle. Please help me if you can.
The mean gray-value obviously depends on the image area you are measuring. Generally the area is defined by the number of contained pixels. If so, the mean gray value is the sum of the values at all pixels divided by the number of pixels. However, the area may be given in scale units (Anlayze >> Set Scale…) which, of course, gives different ...
Hi everyone, Is it possible to convert intensity color scale to concentration color scale in images in ImageJ? For example, I have 8-bit image. I opened it in ImageJ and created color scale using Analyze–>Tools–>Calibration Bar. It created color scale with range 0…255. Is it possible to convert this color scale to mmol/liter color scale, for example 0…150 mmol/liter? I will be very ...
Background is NOT signal from your object. It corresponds to the Back (Black) dot. Draw a rectangle ROI in Fiji and measure the mean signal there. Analyze > Set Measurements… select Mean gray value Then press Analyze > Measure You can then do a global background subtraction: Process > Math > Subtract…. As for any intensity normalization ...