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Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot 'force' its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.
A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. [1] Often used as cloning vectors in genetic engineering, cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. [2] Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage ...
This family includes lambda phage lysozyme and Escherichia coli T4 phage endolysin. [8] Lysozyme helps to release mature phage particles from the cell wall by breaking down the peptidoglycan. The enzyme hydrolyses the 1,4-beta linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of prokaryotic cell walls.
When the Integration Host Factor was first discovered, it was only known for the site-specific recombination of bacteriophage. [4] This is all we knew for a while but through another article, we were able to find that with further research, IHF plays a key role in the scope of physiological processes of E. Coli, including site-specific recombination activities, phage packaging and partitioning ...
Lambdavirus (synonyms Lambda-like viruses, Lambda-like phages, Lambda phage group, Lambda phage) is a genus of viruses in the order Caudovirales, in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There are five species in this genus.
The earliest identified members of the serine recombinase family were known as resolvases or DNA invertases, while the founding member of the tyrosine recombinases, lambda phage integrase (using attP/B recognition sites), differs from the now well-known enzymes such as Cre (from the P1 phage) and FLP (from the yeast Saccharomyces cerevisiae).
Lambda holin S (Lysis protein S of phage lambda, holin S105; TC# 1.E.2.1.1) is the prototype for class I holins. It has 3 TMSs with the N-terminus in the periplasm and the C-terminus in the cytoplasm. Its 107 codon sequence encodes two proteins with opposing functions, the holin, S105, and the holin inhibitor, S107.
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