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An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Selective and differential media reveal characteristics about the microorganisms being cultured on them. This kind of media can be selective, differential, or both selective and differential. Growing a culture on multiple kinds of selective and differential media can purify mixed cultures and reveal to scientists the characteristics needed to ...
An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3).. Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology.
It is particularly useful for the isolation of organisms that cause bacillary dysentery, salmonella strains that cause food poisoning and Salmonella Paratyphi.It is not so selective for Salmonella Typhi.
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
It is a defined, selective and differential medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. After citrate enters a cell through citrate permeases , citrate lyase cleaves it into acetate and oxaloacetate , which is further broken down into carbon dioxide and ...
Chocolate agar showing Francisella tularensis colonies Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, the nonselective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of N. gonorrhoeae, while the selective Thayer–Martin medium on the right, containing ...
Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.