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Cos sequences are ~200 base pairs long and essential for packaging. They contain a cosN site where DNA is nicked at each strand, 12 bp apart, by terminase.This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp.
Comparative efficacy of two fipronil spot-on formulations against experimental tick infestations (Ixodes ricinus) in dogs. Parasitol Res. 2010 Aug;107(3):735-9. doi: 10.1007/s00436-010-1930-y ...
Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains elements required for packaging DNA into λ particles. Under apt origin of replication (ori), it can replicate as a plasmid.
PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. [1] This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.
The first step in Gateway cloning is the preparation of a Gateway Entry clone. There are a few different ways to make entry clone. Gateway attB1 and attB2 sequences are added to the 5' and 3' end of a gene fragment, respectively, using gene-specific PCR primers and PCR amplification. The PCR amplification products are then mixed with a propriet
Home remedies for a cough. There are a few different things you can do to try to treat a cough at home, according to doctors. Use cough drops or lozenges. Try a warm drink like hot water and lemon ...
With you at home, they'll come over to get some affection all day long. Ragdolls are loyal and playful animals and they're large cats! In fact, Ragdolls could be considered gentle giants.
Φ29 is a bacteriophage of Bacillus subtilis with a sequenced, linear, 19,285 base pair DNA genome. [1] Each 5' end is covalently linked to a terminal protein, which is essential in the replication process by acting as a primer for the viral DNA polymerase.
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