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The first impedance-based flow cytometry device, using the Coulter principle, was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter. Mack Fulwyler was the inventor of the forerunner to today's flow cytometers – particularly the cell sorter. [6] Fulwyler developed this in 1965 with his publication in Science. [7]
A Coulter counter played an important role in the development of the first cell sorter, and was involved in the early development of flow cytometry. Some flow cytometers continue to utilize the Coulter principle to provide information about cell size and count.
Flow cytometers operate by aligning single cells using flow techniques. The cells are characterized optically or by the use of an electrical impedance method called the Coulter principle . To detect specific molecules when optically characterized, cells are in most cases stained with the same type of fluorochromes that are used by image cytometers.
This type of hematology analyzer utilizes both Coulter's principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time.
Hydrodynamic focusing solves this problem by building up the walls of the tunnel from fluid, using the effects of fluid dynamics.A wide (hundreds of micrometers in diameter) tube made of glass or plastic is used, through which a "wall" of fluid called the sheath flow is pumped.
Beckman Coulter, Inc. is a Danaher Corporation company that develops, ... the company acquired the Flow Cytometry Business Group of Dako North America, Inc.
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Coulter and CASY counters are much cheaper than flow cytometers, and for applications that require cell numbers and sizes, such as cell-cycle research, they are the method of choice. Its advantage over the methods above is the large number of cells that can be processed in a short time, namely: thousands of cells per second.
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