enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. Primer (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Primer_(molecular_biology)

    The polymerase chain reaction (PCR) uses a pair of custom primers to direct DNA elongation toward each other at opposite ends of the sequence being amplified. These primers are typically between 18 and 24 bases in length and must code for only the specific upstream and downstream sites of the sequence being amplified.

  3. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  4. Polymerase chain reaction optimization - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction...

    Computer simulations of theoretical PCR results (Electronic PCR) may be performed to assist in primer design. [14] Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers will avoid amplifying nonspecific sequences.

  5. Primer binding site - Wikipedia

    en.wikipedia.org/wiki/Primer_binding_site

    A primer binding site is a region of a nucleotide sequence where an RNA or DNA single-stranded primer binds to start replication. The primer binding site is on one of the two complementary strands of a double-stranded nucleotide polymer , in the strand which is to be copied, or is within a single-stranded nucleotide polymer sequence.

  6. Variants of PCR - Wikipedia

    en.wikipedia.org/wiki/Variants_of_PCR

    It involves an initial PCR with primers that have an overlap and a second PCR using the products as the template that generates the final full-length product. This technique may substitute for ligation-based assembly. [8] In colony PCR, bacterial colonies are screened directly by PCR, for example, the screen for correct DNA vector constructs ...

  7. DNA replication - Wikipedia

    en.wikipedia.org/wiki/DNA_replication

    Researchers commonly replicate DNA in vitro using the polymerase chain reaction (PCR). PCR uses a pair of primers to span a target region in template DNA, and then polymerizes partner strands in each direction from these primers using a thermostable DNA polymerase. Repeating this process through multiple cycles amplifies the targeted DNA region.

  8. Random hexamer - Wikipedia

    en.wikipedia.org/wiki/Random_hexamer

    A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.. They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis.

  9. Multiplex polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Multiplex_polymerase_chain...

    The primer design for all primers pairs has to be optimized so that all primer pairs can work at the same annealing temperature during PCR. Multiplex-PCR was first described in 1988 as a method to detect deletions in the dystrophin gene. [1] It has also been used with the steroid sulfatase gene. [2]