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Microbeads serve as the main tool for bio-magnetic separations. A range of patented processes and applications have been developed based on the use of microbeads in academic and industrial research. Microbeads are pre-coupled with a ligand; a biomolecule such as antibody, streptavidin, protein, antigen, DNA/RNA, or other molecule. There are ...
The microbeads are then arrayed in a flow cell for sequencing and quantification. The sequence signatures are deciphered by the parallel identification of four bases by hybridization to fluorescently labeled encoders (Figure 5). Each of the encoders has a unique label which is detected after hybridization by taking an image of the microbead array.
A microbead imaged using scanning electron microscopy. Microbeads are manufactured solid plastic particles of less than one millimeter in their largest dimension [4] when they are first created, and are typically created using material such as polyethylene (PE), polyethylene terephthalate (PET), nylon (PA), polypropylene (PP), and polymethyl methacrylate (PMMA). [5]
Cell sorting is the process through which a particular cell type is separated from others contained in a sample on the basis of its physical or biological properties, such as size, morphological parameters, viability and both extracellular and intracellular protein expression. The homogeneous cell population obtained after sorting can be used ...
The Systems Biology Graphical Notation (SBGN) is a standard graphical representation intended to foster the efficient storage, exchange and reuse of information about signaling pathways, metabolic networks, and gene regulatory networks amongst communities of biochemists, biologists, and theoreticians.
Protein functional analysis is the identification of protein–protein interactions (e.g. identification of members of a protein complex), protein–phospholipid interactions, small molecule targets, enzymatic substrates (particularly the substrates of kinases) and receptor ligands.
In biology, an identification key, taxonomic key, or frequently just key, is a printed or computer-aided device that aids in the identification of biological organisms. Historically, the most common type of identification key is the dichotomous key , a type of single-access key which offers a fixed sequence of identification steps, each with ...
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...