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These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. • PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. • Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step.
The structure of Kurloff cell was identified using light microscopy and periodic-acid Schiff staining. The Kurloff cell has an egg shape and the axis of the cell varies from 8 to 12 μm in length, and 10-25 μm in diameter. [4] [8] The inclusion body, which is round in shape and 1-8 μm in diameter, occupies most of the cell's cytoplasm. [4]
Periodic acid–Schiff–diastase (PAS-D, PAS diastase) stain is a periodic acid–Schiff (PAS) stain used in combination with diastase, an enzyme that breaks down glycogen. PAS-D is a stain often used by pathologists as an ancillary study in making a histologic diagnosis on paraffin -embedded tissue specimens.
M1 macrophages promote atherosclerosis by inflammation. M2 macrophages can remove cholesterol from blood vessels, but when the cholesterol is oxidized, the M2 macrophages become apoptotic foam cells contributing to the atheromatous plaque of atherosclerosis. [57] [58]
[13] Periodic acid-Schiff is a histology special stain used to mark carbohydrates (glycogen, glycoprotein, proteoglycans). PAS is commonly used on liver tissue where glycogen deposits are made which is done in efforts to distinguish different types of glycogen storage diseases.
Joseph Forde Anthony McManus, (July 13, 1911 – March 4, 1980) was a Canadian pathologist who is best known for his formulation of one of the most frequently used stains in histopathology; the McManus Periodic acid-Schiff stain. Joe McManus was a pioneer in the field of Histochemistry during its period of expanding growth and application in ...
Micrograph of the pons using a hematoxylin & eosin-luxol fast blue stain. Coronal section of a mouse brain stained with Hematoxylin & LFB. Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used stain to observe myelin under light microscopy, first developed by Heinrich Klüver and Elizabeth Barrera in 1953. [1]
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining.. Basophilic is a technical term used by pathologists. ...