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Sequence coverage (or depth) is the number of unique reads that include a given nucleotide in the reconstructed sequence. [1] [2] Deep sequencing refers to the general concept of aiming for high number of unique reads of each region of a sequence. [3] Physical coverage, the cumulative length of reads or read pairs expressed as a multiple of ...
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
Therefore, the total number of reads generated in a single experiment is typically normalized by converting counts to fragments, reads, or counts per million mapped reads (FPM, RPM, or CPM). The difference between RPM and FPM was historically derived during the evolution from single-end sequencing of fragments to paired-end sequencing.
In 2012, with cameras operating at more than 10 MHz A/D conversion rates and available optics, fluidics and enzymatics, throughput can be multiples of 1 million nucleotides/second, corresponding roughly to 1 human genome equivalent at 1x coverage per hour per instrument, and 1 human genome re-sequenced (at approx. 30x) per day per instrument ...
The second method employs the use of restriction enzymes and a recognized DNA sequence. [3] The DNA is digested by a particular restriction enzyme, resulting in DNA pieces of varying molecular masses. One of the advantages of this method is that more marker can readily be created simply by digesting more of the known DNA. [3]
One quadrillionth of a second. Pulse time on fastest lasers. svedberg: 10 −13 s: Time unit used for sedimentation rates (usually of proteins). picosecond: 10 −12 s: One trillionth of a second. nanosecond: 10 −9 s: One billionth of a second. Time for molecules to fluoresce. shake: 10 −8 s: 10 nanoseconds, also a casual term for a short ...
Throughput per SMRT cell is around 500 million bases demonstrated by sequencing results from the CHM1 cell line. [ 19 ] On October 15, 2014, PacBio announced the release of new chemistry P6-C4 for the RS II system, which represents the company's 6th generation of polymerase and 4th generation chemistry--further extending the average read length ...
"The metre is the length of the path travelled by light in vacuum during a time interval of 1 / 299 792 458 of a second." 17th CGPM (1983, Resolution 1, CR, 97) 1 / 10 000 000 of the distance from the Earth's equator to the North Pole measured on the circumference through Paris. L kilogram: kg mass