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Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
Therefore, the total number of reads generated in a single experiment is typically normalized by converting counts to fragments, reads, or counts per million mapped reads (FPM, RPM, or CPM). The difference between RPM and FPM was historically derived during the evolution from single-end sequencing of fragments to paired-end sequencing.
In 2012, with cameras operating at more than 10 MHz A/D conversion rates and available optics, fluidics and enzymatics, throughput can be multiples of 1 million nucleotides/second, corresponding roughly to 1 human genome equivalent at 1x coverage per hour per instrument, and 1 human genome re-sequenced (at approx. 30x) per day per instrument ...
In its most restrictive interpretation, this is what may be meant when the term metric unit is used. The unit one (1) is the unit of a quantity of dimension one. It is the neutral element of any system of units. [2] In addition to the unit one, the SI defines 7 base units and associated symbols: The second (s) is the unit of time.
Berkshire Hathaway (NYSE: BRK.A)(NYSE: BRK.B) owns a stock portfolio worth roughly $300 billion with about four dozen individual stocks in it. Legendary stock-picker Warren Buffett himself hand ...
Disintegrations per minute (dpm) and disintegrations per second (dps) are measures of the activity of the source of radioactivity. The SI unit of radioactivity, the becquerel (Bq), is equivalent to one disintegration per second. This unit should not be confused with cps, which is the number of counts received by an instrument from the source.
A U.S. appeals court has rejected British socialite Ghislaine Maxwell's request to revisit its decision upholding her conviction for helping the late financier Jeffrey Epstein sexually abuse ...
REEP5 is expressed across a number of tissues at a relatively high level, with at least 20 Reads Per Kilobase of transcript, per Million mapped reads appearing in adrenal, fat, gall bladder, heart, kidney, prostate, lung, and urinary bladder tissues. [14] Expression is even more highly elevated in brain and thyroid tissues. [14]