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The media used in Kirby–Bauer testing must be Mueller–Hinton agar at only 4 mm deep, poured into either 100 mm or 150 mm Petri dishes. The pH level of the agar must be between 7.2 and 7.4. Bacterial inoculum is prepared by diluting a broth culture to match a 0.5 McFarland turbidity standard , which is equivalent to approximately 150 million ...
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University.
Staphylococcus growth in Baird-Parker (BP) agar.. Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. [1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci. [1]
It is a defined, selective and differential medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. After citrate enters a cell through citrate permeases , citrate lyase cleaves it into acetate and oxaloacetate , which is further broken down into carbon dioxide and ...
Download QR code; Print/export Download as PDF; Printable version; In other projects Wikidata item; Appearance. move to sidebar hide. A microbiological culture of ...
Hoyle's agar is a selective medium that uses tellurite to differentially select Corynebacterium diphtheriae from other upper respiratory tract flora. The medium appears cream to yellow colored, and takes the form of a free-floating powder.
Thioglycolate broth is a multipurpose, enrichment, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes. [1]
Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, note that the non-selective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of Neisseria gonorrhoeae, while the selective Thayer–Martin medium on the right, containing antimicrobials that inhibit the growth of ...